HyMAX™Hybridoma Fusion & Cloning Supplement 20x

貨號:  113004                  

規(guī)格:  100mL

產(chǎn)品描述：

HyMAX™雜交瘤融合與克隆補充劑20x

貨號: 113004
替代名稱：雜交瘤補充劑，雜交瘤克隆因子，HCF，雜交瘤克隆補充物，HCS，雜交瘤克隆融合補體，HCFS，雜交瘤生長補充劑，HGS，雜交瘤融合和克隆加強，雜交瘤融合和克隆補充，HFCS，細(xì)胞培養(yǎng)雜交瘤媒介補充
說明：HyMax™雜交瘤融合和生長補充劑是含有各種細(xì)胞因子，生長因子和激素的條件培養(yǎng)基的專有混合物。在新的雜交瘤發(fā)育過程中配制成替代飼養(yǎng)細(xì)胞。它也可用于從液氮快速回收冷凍細(xì)胞。
融合后細(xì)胞電鍍期間常規(guī)使用飼養(yǎng)細(xì)胞。通常，飼養(yǎng)細(xì)胞為新開發(fā)的克隆提供許多未知的生長因子。這些包括許多涉及同種型轉(zhuǎn)換和B細(xì)胞IgG分泌維持的細(xì)胞因子。從我們多年的雜交瘤發(fā)展經(jīng)驗，我們已經(jīng)配制了這種有條件的媒體，支持更好的生長和更高頻率的IgG生產(chǎn)克隆開發(fā)。測試雜交瘤集落形成效率和IgG分泌的維持。
用法：加入5mL HyMax™20x至95mL您目前用于培養(yǎng)細(xì)胞的工作介質(zhì)，使其占5％。不要使用超過10％的HyMax™。繼續(xù)用這種培養(yǎng)基培養(yǎng)細(xì)胞，直到細(xì)胞生長好幾代。一旦細(xì)胞生長良好，通過逐步將百分比逐步降低到1％，0.5％和0％，可以將培養(yǎng)物從HyMax隔離。
格式：作為無菌過濾的組織培養(yǎng)物測試，20x濃縮液準(zhǔn)備使用溶液。運送到客戶之前切勿凍結(jié)。每個100mL瓶子足以制作2L工作介質(zhì)，足以完成一個完整的雜交瘤開發(fā)項目。
儲存：建議在4°C至8°C的冰箱中儲存，直至6個月內(nèi)使用。它可以制成等分試樣，并在-20°C - 80°C下冷凍保存，長期在12個月內(nèi)使用。避免反復(fù)凍融，這可能會導(dǎo)致活動減少或降水。
穩(wěn)定性：儲存于冰箱中的4°C至8°C或冷凍儲存12個月時6個月。一旦在30天內(nèi)解凍使用，或根據(jù)需要進行等分和儲存。不要重復(fù)凍結(jié)和解凍。
運送：準(zhǔn)備新鮮（30天內(nèi)），并以藍色冰袋液體形式運送。

HyMax與其他產(chǎn)品的第三方獨立比較：

圖-1用于產(chǎn)生IgG產(chǎn)生B細(xì)胞雜交瘤的克隆因子補充物的分析。脾細(xì)胞與SP2 / 0細(xì)胞融合，培養(yǎng)14天，然后分析來自培養(yǎng)物的上清液。通過抗Fc（γ）ELISA進行分析。IgG陽性克隆被鑒定為產(chǎn)生超過25ng / mL IgG的那些。

特點

• 無血清生長添加劑
• 低蛋白含量
• 成分*已知
• zui高克隆效率
• 優(yōu)良的單克隆抗體生產(chǎn)
• 無需喂養(yǎng)細(xì)胞

Roche Applied Science品牌的 11363735001 已停產(chǎn)

HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X
Catalog No.: 113004
Alternate Names: Hybridoma supplement, Hybridoma cloning factor, HCF, hybridoma cloning supplement, HCS, hybridoma cloning fusion supplement, HCFS, hybridoma growth supplement, HGS, hybridoma fusion and cloning booster, hybridoma fusion and cloning supplement, HFCS, Cell Culture Hybridoma Media Supplements
Description: HyMax™ Hybridoma Fusion and Growth Supplement is a proprietary blend of conditioned media containing various cytokines, growth factors & hormones. It is formulated to replace feeder cells during new hybridoma development. It can also be used for fast recovery of frozen cells from liquid nitrogen.
Feeder cells are routinely used during plating of cells after fusion. Typically feeder cells provide many unknown growth factors to the newly developing clones. These includes many cytokines which are involved in isotype switching and maintenance of IgG secretion by B-cells. From our many years of experience with hybridoma development we have formulated this conditioned media that support better growth & higher frequency of IgG producing clone development. It is tested for hybridoma colony forming efficiency and maintenance of IgG secretion.
Usage: Add 5mL of HyMax™ 20x to 95mL of your working media currently used for culturing cells to make 5%. Do not use HyMax™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. Once cells have grown well, culture may be weaned off of HyMax by gradually reducing the percentage in steps to 1%, 0.5% and 0%.
Format: Provided as a sterile filtered, tissue culture tested, 20x concentrate ready to use solution. Never frozen before shipping to customer. Each 100mL bottle is enough to make 2L of working media, enough for doing a complete hybridoma development project.
Storage: It is recommended to store at 4°C - 8°C in refrigerator until use within 6 months. It can be made into aliquots and frozen stored at -20°C - 80°C for long term to use within a 12 month period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.
Stability: 6 months when stored at 4°C - 8°C in refrigerator or 12 months when stored frozen. Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.
Shipping: It is prepared fresh (within 30 days) and shipped in liquid form on blue ice packs.

Third party independent comparisons of HyMax to other products:

Figure-1 Analysis of cloning factor supplements for production of IgG-producing B cell hybridomas. Splenocytes were fused with SP2/0 cells, cultured for 14 days before analysis of supernatant from the cultures. Analysis was performed via anti-Fc (gamma) ELISA. IgG positive clones were identified as those producing more than 25ng/mL IgG.

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